PURIFIED HEPARIN FRACTION, METHOD OF ITS PREPARING AND PHARMACEUTICAL COMPOSITION CONTAINING ON SAID Russian patent published in 1999 - IPC

FIELD: medicine, pharmacy. SUBSTANCE: invention relates to heparins of the low-molecular mass obtained by nitrose depolymerization of the natural heparin and containing nitros compounds at amount less or equal to 5 x 10^-7 mg/mg product. For removal of nitros compounds an aqueous solution of low-molecular mass heparin is treated by ultraviolet rays (wavelength is from 180 to 350 nm) at temperature 5-50 C and pH 3-8. For preparing the purified low-molecular mass heparin an aqueous solution of the natural heparin sodium salt (concentration is 5-15 wt. -%/vol. ) is treated with alkaline metal nitrite as measured 1.5-6.9 wt.-% nitrite to heparin salt taken. The indicated reaction is carried out in the presence of hydrochloric acid at pH from 1 to 5 for time from 20 to 50 min up to negative test for nitro-ions. Obtained product is reduced by addition of sodium boron hydride at amount 0.5-2.0 wt. -% of heparin salt amount taken. An excess of boron hydride is decomposed with hydrochloric acid. Obtained reaction medium is neutralized and the low-molecular mass heparin is precipitated with ethanol as a sodium salt. An aqueous solution of the obtained sodium salt is treated by ultraviolet rays. For preparing nitros compound-containing product at amount less or equal to 5 x 10^-8 mg/mg product the solution treated by ultraviolet rays is fed additionally on anion-exchange column. The indicated column is washed out with water at pH about 7 and the end product is recuperated by precipitation with sodium chloride and ethanol. Pharmaceutical composition based on the low-molecular mass purified heparin shows antithrombosis activity. Method of purification of the low-molecular mass heparin from nitros compounds by ultraviolet irradiation ensures to retain its biological and physical-chemical properties. EFFECT: improved method of preparing. 17 cl, 2 tbl, 2 dwg, 2 ex