METHOD OF PRODUCING NADROPARIN CALCIUM Russian patent published in 2024 - IPC C08B37/10 A61K31/727 

FIELD: chemistry.

SUBSTANCE: invention relates to a method of producing nadroparin calcium, in which sodium heparin is dissolved in water, cooled, pH of the solution is adjusted to 2.45–2.55 and sodium nitrite is added, then bringing the pH of the reaction mass to 9.9–10.7, after which sodium borohydride is added to the solution, then the pH of the reaction mass is brought to 3.8–4.0, after which the reaction mass is brought to pH 6.7–6.9, then ethyl alcohol is added to the solution, stirred and left until the oil settles, the solution is decanted, the remaining oil is stirred in ethyl alcohol until a solid friable precipitate is formed, which is filtered, sodium nadroparin is obtained, then dry sodium nadroparin is dissolved in a phosphate buffer solution and the solution is concentrated, ion exchange is carried out by passing the solution through a weakly acidic cationite to obtain an intermediate product, to which a suspension of calcium hydroxide is added, then pH is adjusted to 6.8 and the solution is evaporated, then calcium nadroparin is lyophilized.

EFFECT: high output of low-molecular weight heparin due to high selectivity of its fractionation using ultrafiltration.

1 cl, 2 ex