METHOD OF QUANTITATIVE DETERMINATION OF AUTOANTIBODIES TO BLOOD SERUM LOW DENSITY LIPOPROTEINS Russian patent published in 1999 - IPC

FIELD: medicine, immunology, therapy. SUBSTANCE: method involves the solid-phase immunoenzymatic analysis where standardization is carried out by affinity antibodies to low density lipoproteins at the known protein concentration of immunoglobulin light chains. Low density lipoproteins isolated from a lot number of donors are used as a binding agent being these lipoproteins are purified from circulating immune complexes "low density lipoproteins + antibody". Goat polyclonal antibodies to the human immunoglobulin light chains labeled with peroxidase are used as the second antibodies to detect autoantibodies to low density lipoproteins by an immunoenzymatic reaction. EFFECT: high specificity and sensitivity of the proposed quantitative method. 2 ex