METHOD FOR CIRCULATING DESIALYLATED LOW DENSITY LIPOPROTEIDS CONTENT ESTIMATION Russian patent published in 2017 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention can be used to assess the content of circulating desialized low-density lipoproteins (cLDLP). The essence of the method is application of a solid phase lectin-immunoenzyme method based on cLDLP binding to 100 μl of RCA120 agglutinin solution in isotonic phosphate buffer (IPB) at a concentration of 30 μg/ml immobilized in plate wells, wells washing with buffered saline with bovine serum albumin (BSA) containing 2 g/l of BSA, introduction of 100 μl of peroxidase-labelled polyclonal antibodies 1 μkg/ml to protein apolipoprotein B, 1 hour incubation at room temperature, introduction of 100 ml of test blood sample to IPB, 2 hours incubation at 20°C, washing, manifestation by pH 4.5 citrate buffer, containing ortho-phenylenediamine and hydrogen peroxide, 30 min incubation at 37°C, reaction stopping by sulfuric acid addition and cLDLP quantification by optical density measuring at a wavelength of 492 nm, which allows to determine the cLDLP content without their preliminary isolation.

EFFECT: invention has higher sensitivity and reproducibility and allows to selectively analyse circulating desialylated low density lipoproteids, thus enables accurate and reliable measurement of circulating desialylated low density lipoproteids concentration without prior isolation of the low density lipoproteins fraction, the method is simple, accessible and rapid in implementation, it allows for screening tests to detect atherosclerotic process presence at the pre-clinical stage and clinical examination of the population.

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