STRAIN OF CULTURED MURINE HYBRIDOMA CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO NUCLEOLUS ANTIGEN ASSOCIATED WITH CELLULAR PROLIFERATION Russian patent published in 2000 - IPC

Abstract RU 2145634 C1

FIELD: biotechnology, medicine. SUBSTANCE: hybridoma is obtained by fusion of murine splenocytes immunized with nuclear fraction from cells of β-lymphoblastoid strain Daudi with murine myeloma cells. Hybridoma produces monoclonal antibodies directed to nucleolus antigen (molecular mass is 35 and 43 kDa) that is detected in proliferating cells of different species, tissue and strain belonging. Invention ensures to study the process of cellular proliferation in malignant diseases and can be used for identification of proliferating cells. EFFECT: strain indicated above, improved method of hybridoma preparing.

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RU 2 145 634 C1

Authors

Malashenko O.S.

Bulycheva T.I.

Dudnik O.A.

Dates

2000-02-20—Published

1998-03-12—Filed