FIELD: medicine.
SUBSTANCE: method includes using a monoclonal A3 antibody for detection of a proliferation marker - nucleolar protein A3 followed by visualisation of positive focuses. A process of proliferation is shown by increasing focuses of A3 antigen localisation from one in G0 phase to 8-10 focuses in an S-period of a cell cycle.
EFFECT: invention allows evaluating effectively a condition of proliferative activity of human lymphocytes.
2 dwg, 1 ex
Title | Year | Author | Number |
---|---|---|---|
IMMUNOCYTOCHEMICAL METHOD FOR ASSESSING PHYTOHEMAGGLUTININ-STIMULATED LYMPHOCYTE BLAST-TRANSFORMATION REACTION (LBTR) | 2012 |
|
RU2514039C2 |
IMMUNOCYTOCHEMICAL ESTIMATION METHOD FOR PROLIFERATIVE LYMPHOCYTE STATE BY EXPRESSION BEHAVIOUR OF C-TERMINAL B23/NUCLEOPHOSMIN PROTEIN FRAGMENT IN INDIRECT IMMUNOFLUORESCENCE TEST | 2010 |
|
RU2438135C1 |
METHOD FOR PROGNOSIS OF PROGRESSING CHRONIC LYMPHOLEUKOSIS BY QUANTITATIVE CONTENT OF NUCLEOLUS PROTEIN B23/NUCLEOFOZMINE IN LYMPHOID CELL LYSATES | 2005 |
|
RU2310201C2 |
STRAIN OF CULTURED CELLS OF MURINE HYBRIDOMA A3 USED FOR OBTAINING MONOCLONAL ANTIBODIES AGAINST HUMAN CELL NUCLEOLUS ANTIGEN | 2005 |
|
RU2296159C2 |
STRAIN OF CULTURED MURINE HYBRIDOMA CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO NUCLEOLUS ANTIGEN ASSOCIATED WITH CELLULAR PROLIFERATION | 1998 |
|
RU2145634C1 |
METHOD FOR PREOPERATIVE DIFFERENTIAL DIAGNOSIS OF BENIGN AND MALIGNANT NODULAR FORMATIONS OF THE THYROID GLAND | 2021 |
|
RU2783304C1 |
METHOD FOR MULTICOLORED IMMUNE-CYTOCHEMICAL DIAGNOSTICS OF PARANEOPLASIA OF CERVIX UTERI | 2020 |
|
RU2753236C1 |
METHOD FOR PRODUCTION OF POLYCLONAL T-CELL VACCINE USEFUL IN TREATMENT OF IMMUNOLOGICAL DISORDERS | 2004 |
|
RU2277422C2 |
METHOD FOR EVALUATING LYMPHOCYTE BLAST-TRANSFORMATION REACTION BY FLOW CYTOMETRY AT SPECIFIC ACTIVATION BY ANTIGEN | 2015 |
|
RU2582395C1 |
METHOD OF DETECTING PROTEINS IN DIFFERENT CELL TYPES OF MAMMALS AND HUMAN USING FLUORESCEIN-5-ISOTHIOCYANATE AT MICROSCOPIC LEVEL | 2013 |
|
RU2547594C2 |
Authors
Dates
2011-10-20—Published
2010-04-01—Filed