METHOD OF BIOLOGICALLY ACTIVE SUBSTANCE PREPARING Russian patent published in 2000 - IPC

FIELD: microbiology. SUBSTANCE: method of preparing biologically active substances is carried out by autolysis of yeast cells of species Saccharomyces cerevisiae followed by separation of liquid fraction of autolyzate from the solid one and addition of part of separated liquid fraction and washing waters from solid fraction to the following cycles of the process of yeast autolysis. Autolysis is carried out at the constant temperature and stirring up to amine nitrogen content in autolyzate from 4.5% to 6%. Washing waters are added up to concentration of dry matters in autolyzate from 15% to 30% at concentration of dry matters in added liquid fraction from 4% to 15%. At final stages of autolysis process the level of dry matters in autolyzate is increased significantly. EFFECT: increased yield of product, decreased energy consumption. 5 cl, 2 ex