BIOLOGICALLY ACTIVE SUBSTANCE (VERSIONS) AND BIOLOGICALLY ACTIVE FOOD ADDITIVE (VERSIONS) Russian patent published in 2007 - IPC A23L1/30 A23L1/305 A23J1/18 A61K36/06 

FIELD: food-processing, biotechnological and medical industry.

SUBSTANCE: yeast-based biologically active substance is produced as chelate complex based on yeast fermentalisate containing at least 60 wt% of peptide fraction with molecular weight exceeding 2.5 kda, at least 14 wt% of free amino acids, at least 8 wt% of amino nitrogen, about 20 wt% of carbohydrates, at least 14 wt% of dry substance. Fermentalisate is produced by fermentative autolysis of Saccaromyces cerevisiae at temperature of 45-60 C and pH of 3-5, cooling to 30-40 C, accompanied by processing with enzyme preparation of lactocanicin. Resultant yeast fermentalisate is cleaned from residual cellular enclosure structures, desalinated and exposed to chelation carried out by preparing 10%-solution of cleaned yeast fermentalisate, followed by microfiltering of solution and obtaining clarified fraction. The latter is treated with 50%-solution of chloride of metals selected from the group consisting of Zn⁺⁺, Cu⁺⁺, Mn⁺⁺, Cr⁺⁺⁺, Fe⁺⁺, with the ratio of yeast fermentalisate solution to aqueous solution of metal salt of (0.5-3):(0.001-2). Acidity of solution is brought to 6.5-7.9 and the latter is held during 0.5-1.5 hours at 15-35 C while being mixed. Resultant solution is subjected to microfiltering for producing of clarified fraction, wherein phosphate buffer of alkaline metal is added and mixed, and mixture is held during 15-50 min at temperature of 15-35 C, followed by microfiltering, obtaining of clarified fraction and drying it so that free metal is present in ready product in an amount not in the excess of 2% by weight of ready product. Biologically active food additive contains said biologically active substance and component selected from the group consisting of hydrocarbons, lipids, vitamins, vitamin-like substances, mineral components, alkaloids, homeopathic means, glycosides, immunoglobulin preparations, phenol compounds, organic acids, enzymes, terpenoids, hormones and hormone-like substances, cytokine, plant products, microbic products, apiculture products, animal products, mineral-containing natural substances, detoxicants, process additives, or mixtures thereof with the following component ratio, wt%: biologically active food additive 10-95; component or mixture of components the balance.

EFFECT: enhanced biological effect, convenient usage, and non-toxicity owing to reduced residue of free metal in product.

5 cl, 4 ex