METHOD OF MUCOVISCIDOSIS DIAGNOSIS Russian patent published in 2000 - IPC

FIELD: medicine, molecular biology. SUBSTANCE: proposed method involves isolation of genome DNA from 5-10 ml of peripheral blood by conventional procedure using proteinase K followed by extraction with mixture phenol/chloroform. For amplification of normal allele direct primer is used: 5'-GGCAC-CATTA-AAGAA-AATAT-CATCTT-3' terminating at 3'-end by trinucleotide CTT that is absent in mutant allele. For amplification of allele with deletion direct primer is used: 5'-GGCAC-CATTA-AAGAA-AATAT-CATTG-G-3' without CTT and terminating by the next trinucleotide TGG at 3'-end. As reverse primer for amplification of both alleles primer is used: 5'-CATTC-ACAGT-AGCTT-ACCCA-3'. EFFECT: simplified, precise method, enhanced safety of diagnosis. 2 cl, 1 dwg, 2 ex