FIELD: medicine.
SUBSTANCE: method involves identifying in the 19th exon of the EGFR gene by polymerase chain reaction (PCR) with using upstream 5'-CTGTCATAGGGACTCTGGAT-3' and downstream 5'-CAGCAAAGCAGAAACTCACAT-3' primers that is followed by measuring a length of an amplified fragment by means of polyacrylamide gel electrophoresis. The L858R substitution in the 21st exon of the gene is detected by allel-specific PCR with using 5'-CACCCAGCAGTTTGGCCA-3', 5'-CACCCAGCAGTTTGGCCC-3' and 5'-GCATGAACTACTTGGAGGAC-3' primers. If observing in patients any of two types of mutations in the EGFR gene, the gefitinib therapy is prescribed.
EFFECT: invention provides high information value and reliability of tumour sensitivity test in the patients suffering lung cancer to gefitinib therapy with underlying simplicity and availability of the method.
2 dwg
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