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2 158 760

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Application

98123923/13, 1998-12-28

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Start date

1998-12-28

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Actual filing date

1998-12-28

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Published

2000-11-10

(72)

Inventor

Nagieva F.G.Gruzdev K.N.Obukhov I.L.Barkova E.P.Nikulina V.G.

(73)

Holder

Vserossijskij Gosudarstvennyj Nauchno-Issledovatel'Skij Institut Kontrolja, Standartizatsii I Sertifikatsii Veterinarnykh Preparatov

STRAIN OF HYBRID CULTURED ANIMAL CELLS OF MUS MUSCULUS L USED FOR PRODUCTION OF MONOCLONAL ANTIBODIES TO CHLAMYDIA Russian patent published in 2000 - IPC

Abstract RU 2158760 C2

FIELD: veterinary biotechnology. SUBSTANCE: strain Chla-K-1 is obtained by fusing mouse myeloma cells NSO with splenocyte cells of BALB/c mouse line immunized by elementary corpuscles Chlamydia psittaci prepared from strain "K-1" feline chlamydiosis conjunctivitis exciter. Hybridoma Chla-K-1 produces genus-specific Gl isotype monoclonal antibodies reacting with different agents exciting feline, cheep, fur-bearing animal, and human chlamydiosis. Activity of antibodies in cultural fluid ranges from 1:2000 to 1:6000, in ascitic fluid from 1:200000 to 1:1000000. Antibodies are revealed in immunoenzyme test involving immuno-fluorescence. EFFECT: enabled preparation of express diagnosticum for detection of Chlamydia genus detection. 1 tbl, 4 ex

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RU 2 158 760 C2

Authors

Nagieva F.G.

Gruzdev K.N.

Obukhov I.L.

Barkova E.P.

Nikulina V.G.

Dates

2000-11-10—Published

1998-12-28—Filed

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