FIELD: biochemical methods. SUBSTANCE: fat globules are isolated from animal milk, washed, and treated with Triton X-100 (detergent). Resulting fat globule membrane solubilizate is purified and subjected to affinity chromatography to isolate receptors. As active sorbent, prolactine immobilized on epoxyactivated TSK gel is used. Sorbent is prepared by incubation in buffer with pH 7,4-9,0. EFFECT: increased purity of receptors and enhanced efficiency of their production. 4 dwg, 5 ex
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