METHOD OF STIMULATION OF PROLIFERATIVE ACTIVITY OF LYMPHOCYTES Russian patent published in 2001 - IPC

FIELD: medicine. SUBSTANCE: splenocytes are placed in medium RPMI-1640 containing additionally 5% inactivated human serum of 4-th group, 2 mM of L-glutamine, 10 mM of buffer HEPES, 5 x 10^-5 M of 2-mercaptoethanol and 50 mcg/ml of gentamycin is subjected for effect of argon plasma obtained at current power value 30 A, voltage 20 V and gas consumption 1 l/min. Effect is carried out for 30 s from the distance 20 cm from plasmotron nozzle. Method ensures to carry out the local effect on lymphocytes functions both in cultured cells and in the body. Invention can be used in clinic practice. EFFECT: improved method of stabilization, decreased time of physical factor effect on cells. 1 tbl, 2 ex