BIOLOGICALLY ACTIVE PREPARATION SHOWING IMMUNOMODULATING PROPERTIES Russian patent published in 1995 - IPC

FIELD: medicine. SUBSTANCE: preparation is a mixture of substances involving interleukin-2 at concentration 1 wt.-% not above, bioactive cytokines at concentrations corresponding natural ratio, mixture of α- and b-chains of hemoglobin, impurities of nonidentified structure prepared from mammal mononuclear blood cells by their stimulation with activating addition of Ca-ionophore (concentration is 1-1000 ng/ml) and phorbol-12-myristate-13-acetate (concentration is 1-100 ng/ml) followed by cultivation and isolation from supernatant by a single step of liquid chromatography. Preparation can contain fetuin (concentration is 0.5-10 wt.-%) in the case of addition of activating additions of fetal serum to the composition. Preferable cell concentration in cultural fluid is 0.5-10 mln/ml, desirable cultivation time is 1-120 hr. Eluent used at liquid chromatography is 0.01 M trifluoroacetic acid solution or 15, 30 and 60% acetonitrile solution in 0.01 M aqueous trifluoroacetic acid solution. After separation of supernatant mononuclear cells were preferably to be stimulated repeatedly with activating additions. Proposed preparation shows the following advantages: high immunomodulating activity, the presence of natural carriers (a- and b -chains of hemoglobin), multicomponent nature of preparation excludes the necessity of selection and addition of recombinant cytokines, simple technology of preparing, absence of antibiotics in the process of its preparing that increases raw activity, possibility for repeated use of mononuclear cells. EFFECT: enhanced quality of preparation. 6 cl, 5 dwg