FIELD: medicinal microbiology. SUBSTANCE: accumulation of bacteria of genus Lactobacillus from clinical material is performed at 39 C in selective liquid nutrient enrichment medium in the following ratio of medium components, g/l: dry enzymatic hydrolyzate of defatted milk, 30.0 ± 3.0; glacial acetic acid, 3.4 ± 0.1; concentrated yeast autolysate, 110.0 ± 10.0; agar, 0.8; distilled water, up to 1 l; 20% sodium hydroxide solution, to pH value of medium 5.4 ± 0.1. Then lactobacilli are isolated in solid selective nutrient medium in the following ratio of medium components, g/l: dry enzymatic hydrolyzate of defatted milk, 30.0 ± 3.0; glacial acetic acid, 3.4 ± 0.1; concentrated yeast autolysate, 110.0 ± 10; agar, 22.5 ± 2.5; distilled water, up to 1 l; 20% sodium hydroxide solution, to pH value of medium 5.4 ± 0.1. Invention can be used for selective isolation of bacteria of genus Lactobacillus from clinical material (feces, vaginal secretion). EFFECT: enhanced effectiveness, simplified method, improved diagnosis of gastroenteric diseases. 1 cl, 1 ex
