METHOD OF HIGHLY PURIFIED MONOCOMPONENT INSULIN PREPARING Russian patent published in 2002 - IPC

Abstract RU 2186581 C2

FIELD: medicinal industry, preparative biochemistry, hormones. SUBSTANCE: invention relates to preparing highly purified monocomponent insulin of different origin. Crude insulin is subjected for two procedures of column reverse-phase chromatography: at pH 1.5-4.5 and at pH 6.5-9.5. Purification is carried out on nonpolar sorbents exhibiting mean pores size 100-500 and particles size from 10 to 100 mcm. Aqueous-organic solvents containing ionic modifying agents are used as eluents. Process of chromatography treatment is carried out at temperature 5-30 C. Method provides preparing insulin of high quality containing 98.5% of basic substance, not less, 0.5% of desamido-A21-insulin, not above, 1.0% of unidentified impurities, not above, 1.0 mln^-1 of proinsulin, not above. EFFECT: improved method of purification, high quality and purity of insulin. 15 cl

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RU 2 186 581 C2

Authors

Lutsiv V.R.

Dates

2002-08-10—Published

2000-03-28—Filed