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2 447 149

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IPC

C12N15/00(2006-01-01)

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Application

2011111205/10, 2011-03-24

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Start date

2011-03-24

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Actual filing date

2011-03-24

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Published

2012-04-10

(72)

Inventor

Kostetskij Igor' Evgen'EvichLisovskij Igor' LeonidovichLutsiv Vladimir RomanovichMarkeeva Natal'Ja Vladimirovna

(73)

Holder

Vinsort Menedzhement Ink

RECOMBINANT PLASMID DNA pMSIN4, CODING HYBRIDE POLYPEPTIDE - HUMAN INSULIN PRECURSOR, BL21(DE3)VpMSIN4-PRODUCER STRAIN OF RECOMBINANT HUMAN INSULIN, METHOD FOR PRODUCING RECOMBINANT HUMAN INSULIN Russian patent published in 2012 - IPC C12N15/00

Abstract RU 2447149 C1

FIELD: medicine.

SUBSTANCE: what is described is a recombinant plasmid pMSIN4 for the expression of recombinant human insulin having the size of 5495 base pairs, consisting of the following elements: kanamycin resistance gene (Kang, 813 base pairs); cloned gene (225 base pairs) coding an insulin precursor protein and containing a pre-peptide consisting of 21 amino acid residues (2.46 kDa); a T7 promoter (17 base pairs); arginine codon (CGC) binding the sequences coding insulin B chain and A chain; a replication site (ColEl origin, 588 base pairs). What is presented in the BL21 (DE3)/pMSIN4 strain (deposited in the Czech Collection of Microorganisms, Masaryk University, Brno, Czech Republic, No. CCM 7884) is produced by transformation of E.coli cells of the BL21(DE3) strain by the recombinant plasmid pMSIN4. What is described is a method for producing recombinant human insulin involving cultivation of the presented producer strain.

EFFECT: invention provides higher quality and yield of the end product - recombinant human insulin, simplified method for producing recombinant human insulin in industry.

4 cl, 1 dwg, 1 tbl

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RU 2 447 149 C1

Authors

Kostetskij Igor' Evgen'Evich

Lisovskij Igor' Leonidovich

Lutsiv Vladimir Romanovich

Markeeva Natal'Ja Vladimirovna

Dates

2012-04-10—Published

2011-03-24—Filed