FIELD: biotechnology, biochemistry. SUBSTANCE: method involves the successive combination of reversed-phase and anion-exchange methods on chromatography unit of low pressure. Method involves loading and elution processes of components to be separated by frontal-displacing regime. Highly purified insulin has, %: 7% basic substance, not less 7; proinsulin, not above 0.001; high-molecular proteins, not more, 1; insulin-like impurities, not more 1. The yield of end product is not less than 70%. EFFECT: improved method of purification, enhanced quality of product. 13 cl, 1 ex
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