TRUNCAL NERVE CELLS, CELLS CULTURE AND BIOTRANSPLANT FOR ALLOTRANSPLANTING AND METHOD FOR PREPARING THE BIOTRANSPLANT Russian patent published in 2002 - IPC

Abstract RU 2191388 C1

FIELD: medicine. SUBSTANCE: cell culture is produced from anterior brain tissue taken at the first trimester of pregnancy from abortive human embryos or from periventricular brain tissue of abortive human fetuses during 15-20 weeks of gestation. Biotransplant has the truncal cells selectively separated and reproduced to the number of 10⁷-10⁹ of pluripotent undifferentiated cells with neurospheres. To prepare the biotransplant, human embryo brain tissues are suspended and cultured in media containing no serum with growth factors being added and multiple medium substitution. The cultured cell aggregates are dissociated and truncal cell neurospheres are separated. EFFECT: enhanced effectiveness of treatment; avoided adverse transplantation side effects. 7 cl

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RU 2 191 388 C1

Authors

Repin V.S.

Rzhaninova A.A.

Shamenkov D.A.

Dates

2002-10-20—Published

2001-12-18—Filed