TRANSCRIPTION SYSTEM IN HIGHER PLANT PLASTID ENCODED BY NUCLEAR DNA Russian patent published in 2002 - IPC

FIELD: molecular biology, biochemistry. SUBSTANCE: DNA structures for constant plastid transformation comprise transforming DNA with orienting segment, marker gene determining selecting phenotype to vegetable cells containing transformed plastids and cloning site for insertion of additional DNA encoding foreign gene to be interested. The presence of 5'- promoter element in DNA structure recognized and transcribing by plastid RNA-polymerase encoded by nuclear DNA and additional 5'-promoter element that is recognized and transcribed by plastid RNA- polymerase encoded by plastid DNA alleviates transformation of many plants significantly and ensures to carry out tissue-specific expression of DNA used for transformation of plastids of multicellular plants. EFFECT: valuable properties of system. 11 cl, 13 dwg, 3 tbl