CHROMATOGRAPHY METHOD OF ISOLATION AND PURIFICATION OF PROTEINS, PEPTIDES AND THEIR COMPLEXES Russian patent published in 1998 - IPC

Abstract RU 2122549 C1

FIELD: biochemistry, medicine, endocrinology. SUBSTANCE: human insulin obtained from preproinsulin where natural methionine located between leader sequence is substituted with arginine is isolated and purified by ion-exchange chromatography. Method ensures to obtain insulin without toxic reagents using. EFFECT: simplified technology, high yield and purity of product. 4 cl, 1 ex

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RU 2 122 549 C1

Authors

Uvarov V.Ju.

Nechaev V.N.

Markin S.S.

Semenov M.P.

Sergienko V.I.

Dates

1998-11-27—Published

1997-12-29—Filed