FIELD: biotechnology, microbiology. SUBSTANCE: method involves culturing Azotobacter chroococcum 7B on nutrient medium containing excess of sucrose as the basic carbon source and sodium acetate as additional carbon source in the following ratio of concentrations of basic and additional carbon source from 40:2 to 40:7. Concentration of sodium acetate is measured by the formula: C = β where C is concentration of sodium acetate , g/l; M is molecular mass, kDa. Method provides to prepare polymer with the required molecular mass in culturing a single producer. EFFECT: improved method of polymer preparing.
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Authors
Dates
2003-03-27—Published
2001-10-18—Filed