METHOD OF LEPTOSPIRA CULTURING Russian patent published in 2003 - IPC

Abstract RU 2202608 C2

FIELD: microbiology, microbiological industry, biotechnology. SUBSTANCE: invention relates to methods for preparing nutrient media used for culturing microorganisms of leptospira family. Culturing of leptospira is carried out in aqueous-serum medium containing enzymatic muscle hydrolyzate in the required amount of chemical compounds at pH 7.4-7.6. Culturing of leptospira is carried out according to existing instructions at temperature 28-30 C for 5-7 days. In experiments for culturing the following industrial strains of leptospira of serological groups are used: Grippotyphosa, Pomona, Tarassovi, Interohemorrhagia, Canicola, Seiro. Invention can be used in industrial and laboratory culturing leptospirae with aim of preparing vaccines for prophylaxis of leptospirosis in agriculture animals and humans. EFFECT: enhanced effectiveness and economy of medium. 4 tbl, 4 ex

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RU 2 202 608 C2

Authors

Sit'Kov V.I.

Zaerko V.I.

Surmilo A.P.

Stefanidi G.N.

Tutov I.K.

Popashenko R.I.

Kharchenko V.G.

Dates

2003-04-20Published

2000-07-27Filed