FIELD: veterinary science, microbiology. SUBSTANCE: invention relates to methods for preparing allergens used in diagnosis of diseases caused by microorganisms from genus Rhodococcus. Method involves usage of active protein preparing from cultural fluid of Rhodococcus bronchialis as a diagnostic allergen that provides high precision of diagnosis carrying out. Method for preparing allergen is carried out by precipitation of active protein from Rhodococcus bronchialis cultural fluid using sodium chloride. Method is simple, effective and provides preparing the specific allergen. EFFECT: improved preparing method, valuable properties of allergen. 2 cl, 5 tbl
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