METHOD FOR DETECTING ANTIBODIES TO TRANSGLUTAMINASE USABLE FOR DIAGNOSING CELIAC DISEASE Russian patent published in 2003 - IPC

FIELD: medicine. SUBSTANCE: method involves applying transglutaminase antigen conjugated to colored substance like particles of colloid gold or colored latex for determining IgA and IgG isotypes presence in blood. The conjugated antigen is bound to inert fiber carrier from it is releasable as liquid in biological way. Antibodies in the sample make reaction with the conjugated antigen by forming immune complex migrating along nitrocellulose or nylon carrier membrane having pores of the size allowing the reagents to move on the membrane until it enters in reaction with the same transglutaminase antigen immobilized on the membrane in reaction zone. The immune complex becomes fixed on the reaction place as a result of this reaction and color signal arises. Visual signal occurrence in the reaction zone is interpreted as positive antibody detection test result for antibody to transglutaminase in the sample. EFFECT: accelerated and simplified antibody detection. 5 cl, 3 dwg