FIELD: medicine.
SUBSTANCE: method comprises obtaining of a sample from the said animal. The first signal obtained by binding of the first part of the sample to the whole wheat antigen containing alcohol-soluble and water-soluble wheat antigens is measured. The second signal obtained by binding of the second part of the sample to a gliadin antigen comprising deamidated α-gliadin. The third signal obtained by binding of the third part of the sample to an antigen selected from the group consisting of: (1) wheat germ agglutinin; (2) gluteomorphine; (3) glutenin; (4) deamidized glutenin; and (5) prodinorphin is also measured, and the results of this test are analyzed. The obtained results indicate the presence of celiac disease in case if the said first signal indicates the presence of IgA or IgG against the said wheat antigens, the said second signal indicates the presence of IgA or IgG against the gliadin antigen, and the said third signal indicates the presence of IgA or IgG against at least one antigen selected from the group consisting of glutotoxin, glutenin, deamidated glutenin, and prodinorphin. The obtained results indicate the presence of gluten immunoreactivity and sensitivity in case if the said first signal indicates the presence of IgA or IgG against the said wheat antigens, the said second signal indicates the presence of IgA or IgG against the gliadin antigen, with the exception of deamidated -gliadinα, and the said third signal indicates the presence of IgA or IgG against at least one antigen selected from the group consisting of glutenomorphin, glutenin, or indicative of the absence of IgA or IgG against deamidized glutenin. The group of inventions also relates to a version of the diagnostic method, to a test plate for use in the diagnostic method.
EFFECT: use of this group of inventions allows to differentiate gluten immunoreactivity and sensitivity, latent celiac disease, Crohn's disease and other intestinal pathologies from classical celiac disease.
24 cl, 7 dwg, 8 tbl, 4 ex
