FIELD: experimental medicine. SUBSTANCE: method for culturing involves incubation of cellular material in liquid nutrient medium in chamber at 37 C. Before incubation cellular material is placed on semi- permeable filter fitted into chamber that is filled with liquid nutrient medium preliminary. In the process of incubation the directed moving liquid nutrient medium is carried out using pump at the rate 8-12 cm3/min from collector of nutrient medium and inversely. Incubation is carried out for 72 h. Invention provides the reduced culturing time and increased amount of cells obtained. EFFECT: improved culturing method. 1 dwg
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Authors
Dates
2004-01-10—Published
2002-05-07—Filed