FIELD: medicine.
SUBSTANCE: invention relates to medicine, namely to ophthalmology, and aims at treating endothelial epithelial corneal dystrophy (EECD). Method involves introducing a suspension of autologous blood mononuclear leukocytes into the posterior chamber of the eye until it replaces the aqueous humor in the anterior chamber by performing paracentesis near the corneal limbus. For this purpose, paracentesis is performed twice at 7 and 11 o'clock with the puncture size of 1-2 mm, wherein an irrigation system in the form of a cannula used to supply sterile air at the pressure of up to 0.2 atm into the anterior chamber is installed into one of the punctures; and 96-98 % cell suspension of autologous blood mononuclear leukocytes is syringed once through the second paracentesis into the anterior chamber between the inner surface of the cornea and air bubble. Then the irrigation system is removed and edges of paracenteses are hydrated. Suspension of autologous blood mononuclear leukocytes is obtained by extraction using the density gradient fractionation method and separating solution of Ficoll-verografin.
EFFECT: use of the invention enables to reduce the length of treatment and complications associated with excessive involvement of ocular tissues, corneal oedema.
1 cl, 10 dwg
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Authors
Dates
2016-11-10—Published
2015-07-30—Filed