FIELD: microbiology, ecology.
SUBSTANCE: invention relates to methods for preparing streptomycin-resistant variants of anthrax pathogen. Method involves stratifying membrane filters on surface of solid nutrient medium. Breeding streptomycin-resistant strain of B. anthracis with M. xanthus is carried out by mixing cell biomass of these microorganisms and seeding this mixture on surface of membrane filters stratified on surface of nutrient medium. Separate seeding of biomass B. anthracis and M. xanthus is used as control. Seeding is incubated in thermostat at 35 C for 24 h. Then replication (fingerprints) of grown populations is carried out in selective medium containing streptomycin in the concentration 100 mcg/ml and incubated colony fingerprints at 35 C for 48-72 h. Method provides detecting and preparing streptomycin-resistant variants of anthrax pathogen from symbiotic mixture of cells B. anthracis and slipping microorganism M. xanthus.
EFFECT: improved preparing method.
1 tbl, 3 ex
| Title |
Year |
Author |
Number |
| STRAIN OF BACTERIA B anthracis KM104-PLAMID-FREE pXO1-Тох, pXO2-Cap AVIRULENT, рВС16 TETRACYCLINE-RESPONSIVE Tet, STREPTOMYCIN-DEPENDENT Str TO BE USED IN GENETICS |
2008 |
- Bulantsev Aleksandr Leonidovich
- Alekseev Vladimir Valer'Evich
- Lipnitskij Anatolij Vasil'Evich
- Dandina Ol'Ga Vladimirovna
|
RU2384614C1 |
| METHOD FOR SELECTION OF SINGLE-PLASMID VARIANTS OF BACILLUS ANTHRACIS IN S-FORM FROM MIXED (S-R)-POPULATION USING MYXOCOCCUS XANTHUS |
2003 |
- Bulantsev A.L.
- Elizarov V.V.
- Lipnitskij A.V.
|
RU2233886C1 |
| STREPTOMYCIN RESIDUAL ACTIVITY TEST ENSURED BY SPORE TEST CULTURES OF STRAIN Bacillus anthracis Davies "R" БАЛ №31 Str IN NUTRIENT MEDIUMS, BIOLOGICAL TISSUES AND FLUIDS |
2008 |
- Bulantsev Aleksandr Leonidovich
- Alekseev Vladimir Valer'Evich
- Lipnitskij Anatolij Vasil'Evich
- Dandina Ol'Ga Vladimirovna
|
RU2384622C1 |
| METHOD FOR INDUCTION OF HEMOLYTIC ACTIVITY OF Bacillus anthracis |
2004 |
- Bulantsev A.L.
- Elizarov V.V.
- Lipnitskij A.V.
|
RU2265666C1 |
| STRAIN BACILLUS SUBTILIS TPAXC SHOWING RESISTANCE TO TETRACYCLINE, RIFAMPICIN, AMPICILLIN, CHLORAMPHENICOL, STREPTOMYCIN AND EXHIBITING ANTIBACTERIAL ACTIVITY WITH RESPECT TO PATHOGENIC MICROORGANISM SPECIES |
1997 |
- Bulantsev A.L.
- Tikhonov N.G.
- Lipnitskij A.V.
|
RU2118364C1 |
| STRAIN OF BACILLUS SUBTILIS TPAC SHOWING RESISTANCE TO TETRACYCLINE, RIFAMPICIN, AMPICILLIN, STREPTOMYCIN AND EXHIBITING ANTIBACTERIAL ACTIVITY WITH RESPECT TO MICROORGANISM PATHOGENIC SPECIES |
1997 |
- Bulantsev A.L.
- Tikhonov N.G.
- Lipnitskij A.V.
|
RU2122026C1 |
| STRAIN FRANCISELLA TULARENSIS FOR PREPARING LIVE VACCINE AGAINST TULAREMIA |
1987 |
- Kormilitsyna Marina Il'Inichna
- Marakusha Boris Ivanovich
- Petrovskaja Valentina Georgievna
- Meshcherjakova Irina Sergeevna
|
SU1839960A1 |
| STRAIN BACILLUS SUBTILIS PAC SHOWING RESISTANCE TO RIFAMPICIN, AMPICILLIN AND STREPTOMYCIN AND ANTIBACTERIAL ACTIVITY WITH RESPECT TO PATHOGENIC MICROORGANISMS SPECIES |
1997 |
- Bulantsev A.L.
- Tikhonov N.G.
- Lipnitskij A.V.
|
RU2115725C1 |
| METHOD OF PREPARING ATTENUATED IMMUNOGENIC STRAINS OF PASTEURELLA MULTOCIDA |
1995 |
- Stepanshin Ju.G.
- Manzenjuk I.N.
- Svetoch Eh.A.
- Gusev V.V.
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RU2103355C1 |
| METHOD OF RECIPIENT SELECTION FOR REALISATION OF TRANSDUCTION BACILLUS ANTHRACIS AND CLOSELY RELATED BACILLI |
2007 |
- Bulantsev Aleksandr Leonidovich
- Lipnitskij Anatolij Vasil'Evich
- Kurilov Viktor Jakovlevich
|
RU2350651C1 |
