FIELD: medicine; microbiology.
SUBSTANCE: efficiency of the transducing phage crops multiplied on the strain-donor, and strains of the future recipients. Quality of the recipient is checked at statement of an intraspecific and interspecific transduction, by addition of 1 ml transducing phage of various concentration to 1 ml recipient cultures B.anthracis, B.thuringiensis and B.cereus with the various characteristic of efficiency of the transducing phage crops, and the control - 1 ml recipient culture admix about 1 ml of a phage buffer. Incubate during 20 minutes at 33°C in a shaker at 100 sh. rot./minute, then in all assays it is brought on 1 ml of a preparation of cellular receptors of corresponding recipients. Again incubate during 30 minutes at 33°C. Then in each assay bring on 1 ml of corresponding receptors and inoculate on 0.2 ml from skilled and control assay on the selective Hottinger mediums, grown TetR colonies - transductant are calculated in 48-72 hours after incubation of crops at 35°C. The recipient is considered optimum for transduction if efficiency of the transducing phage crops is peer 0.5-1.0 (relation of an indicator of concentration transducing phage received on the strain-donor to an indicator of concentration received on the selected strain-recipient).
EFFECT: selection of optimum recipients for realisation of a transduction from an available collection of strains Banthracis and closely related bacilli.
1 dwg, 2 tbl, 2 ex
