METHOD FOR PRODUCTION OF CHITOSAN OLIGOSACCHARIDE (VARIANTS) Russian patent published in 2005 - IPC

FIELD: medicine.

SUBSTANCE: chitosan oligosaccharides are obtained by enzymatic hydrolysis of 10 % chitosan solution for 30-50 min. Then chitosan and chitosanase are additionally added into solution to produce total chitosan concentration of 10 mass %, and fermentation is carried out up to total time of 20-24 h. In another embodiment beforehand enzymatic hydrolysis of 5 % chitosan solution for 30-50 min is carried out, then chitosan and chitosanase are additionally added into solution to produce total chitosan concentration of 10 mass %, and fermentation is carried out for 30-50 min. After that chitosan and chitosanase are added into solution again to produce total chitosan concentration of 15 mass %, and fermentation is carried out up to total time of 20-24 h. In the third embodiment into aqueous solution containing organic acids simultaneously chitosan in amount of 10-15 mass % and chitosanase are added and fermentation is carried out for 20-24 h. In all variants mixture of acetic and ascorbic acids, acetic acid, mixture of acetic and succinic acids, mixture of ascorbic and succinic acids are used as organic acids. Method of present invention makes it possible to increase target product yield by 2-3 times without losses of characteristics thereof.

EFFECT: method of improved yield; product of high quality.

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