FIELD: biotechnology, in particular for biochemical, immunological and biological investigation and for production of insulin pharmaceuticals.
SUBSTANCE: semisynthetic human insulin is prepared by transpeptidation of swine insulin in presence of trypsin in mass ratio trypsin/swine insulin of 1:300-1000 and molar excess threonyn di-tert-butyl ester in relation to swine insulin, in aqueous-organic medium. Reaction is inhibited by pH reducing and reaction mixture dilution with water by 2-3 times. Obtained human insulin ester is purified by HPLC followed by providing of human crude insulin crystals and repeated HPLC-purifying. Method of present invention makes it possible to increase human insulin ester yield up to 98 % and decrease by-product contamination in finished product up to 0.9 %.
EFFECT: method of improved yield; product of improved purity.
6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING READY MEDICINAL FORMULATION OF INSULIN WITH SHORT EFFECT | 2002 |
|
RU2261107C2 |
| METHOD FOR PRODUCTION OF INSULIN DRUG OF DURABLE ACTION | 2002 |
|
RU2252782C2 |
| METHOD FOR PREPARING HUMAN INSULIN COMBINED PREPARATION | 2002 |
|
RU2261108C2 |
| HUMAN INSULIN DRUG OF DURABLE ACTION | 2003 |
|
RU2252753C2 |
| HUMAN INSULIN DRUG OF SHORT ACTION | 2003 |
|
RU2252752C2 |
| METHOD FOR PREPARATION OF INSULIN COMPOUNDS | 2002 |
|
RU2376379C2 |
| RECOMBINANT PLASMID DNA pMSIN4, CODING HYBRIDE POLYPEPTIDE - HUMAN INSULIN PRECURSOR, BL21(DE3)VpMSIN4-PRODUCER STRAIN OF RECOMBINANT HUMAN INSULIN, METHOD FOR PRODUCING RECOMBINANT HUMAN INSULIN | 2011 |
|
RU2447149C1 |
| METHOD OF PRODUCING HUMAN INSULIN OF INS-THR-OH FORMULA, OR ITS DERIVATIVES | 0 |
|
SU1554766A3 |
| METHOD OF PURIFICATION OF INSULIN AND/OR ITS DERIVATIVES | 1991 |
|
RU2037500C1 |
| RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN | 2006 |
|
RU2354702C2 |
