FIELD: analytical methods in medicine.
SUBSTANCE: invention relates to use of fibrin monomer to determine tissue activator of plasminogen and/or its inhibitor. Method consists in treatment of fibrinogen with thrombin-like enzyme ancistron isolated from poison of snake Agkistrodon halys inhabiting Russian Federation areas followed by dissolution of resulting crosslinked fibrin via dialysis against 0.001 N HCl to give transparent solution containing purified de-AA fibrin monomer.
EFFECT: increased accessibility and reduced expenses on utilized thrombin-like enzyme, increased plasminogen-stimulating effect of de-AA fibrin monomer.
3 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
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