METHOD FOR DETERMINING TISSULAR PLASMINOGEN ADJUVANT Russian patent published in 2005 - IPC

FIELD: medicine.

SUBSTANCE: method involves incubating sample with plasminogen, plasminogen-to-plasmin transformation stimulator under the tissular plasminogen adjuvant action and chromogenic synthetic plasmin substrate and determining tissular plasminogen adjuvant activity from substrate hydrolysis product concentration colored with the plasmin. Lys-plasminogen pretreated with pancreatic trypsin inhibitor or Glu-plasminogen is used as the plasminogen. Specific HCO-Ala-Phe-Lys-p-ntroanilide is applied as the plasmin substrate. Soluble fibrin-monomer produced by means of thrombine-like enzyme ancistrone from snake poison of is applied as the plasminogen-to-plasmin transformation stimulator under tissular plasminogen adjuvant action. Incubation is carried out at pH=7.0-8.5. The most optimum concentration for Lys- or Glu-plasminogen is equal to 0.3 mcM, and it is equal to 0.6 mM for the specific HCO-Ala-Phe-Lys-p-ntroanilide substrate, and 0.025 mg/ml for the fibrin-monomer.

EFFECT: enhanced sensitivity and accuracy of the method.

2 cl, 9 dwg