THE PROLONGED THROMBOLYTIC AGENT AND A METHOD OF ITS PREPARING Russian patent published in 1998 - IPC

FIELD: medicine. SUBSTANCE: invention relates to acylated derivatives of enzymes - 4-(N,N,N-trimethylamino)-cinnamoyl derivatives of fibrinolytic enzymes: urokinase, plasminogen and plasmin tissue activators, activating complexes of plasmin-and plasminogen-streptokinase. Method of preparing these acylated derivatives of enzymes involves acylation of fibrinolytic enzymes by serine residue in an active center with an excess of 4-(N,N,N-trimethylamino)-cinnamic acid 4-nitrophenyl ester. Acylation is carried out at 0-25 C and pH 7.0-8.5 for 10-70 min in the absence of an organic solvent. The obtained acylenzymes are separated from unreacted acylating agent and the formed 4-nitrophenyl ester by gel-filtration or ultrafiltration and protein fractions are lyophilized. The prepared agents can be used for treatment of patients with thromboembolic diseases. EFFECT: improved method of preparing. 2 cl, 1 tbl, 7 ex