FIELD: medicine.
SUBSTANCE: as initial material one should apply urine of pregnant women at late terms of pregnancy or that of gestosis-suffering pregnant women at late terms of pregnancy. Saline protein fractioning should be carried out with ammonium sulfate of 40% saturation, this solution should be exposed for 8-10 h followed by centrifuging at 7000 rot./min for 2025 min. Residue diluted with 0.15 M sodium chloride solution should be salted out due to dialysis against 0.025 M phosphate buffer at pH - 7.4. The method provides more simplified technique and shortened terms for obtaining the preparation desired.
EFFECT: higher efficiency.
1 cl, 1 dwg, 6 ex
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