METHOD FOR OBTAINING BRUCELLOSIS DIAGNOSTIC KIT Russian patent published in 2006 - IPC A61K39/10 G01N33/569 

FIELD: medicine, veterinary science.

SUBSTANCE: the present innovation deals with obtaining diagnostic preparations and could be applied for obtaining a diagnostic kit to detect antibodies to brucellosis antigen in agglutination micro-reaction (AMR), in tube agglutination reaction (AR) and in lamellar agglutination upon a slide. The innovation deals with washing microbial mass Brucella abortus 19 BA with 0.9%-sodium chloride solution, moreover, concentration of microbial mass should achieve about 20-30 bln. m.c/ml, inactivation of microbial suspension with 1%-formalin solution performed at 22° C for 2 h, washing due to centrifuging twice at 7000 rot./min for 30 min, staining microbial suspension with 0.04%-Karaci hematoxylin solution to be added at equal volume to the residue obtained after adding 0.9%-sodium chloride solution up to initial volume, then the mixture should be mixed, heated at 56° C for about 16-18 h. After staining the suspension should be centrifuged at 2000 rot./min for 15 min, then supernatant should be centrifuged at 7000 rot./min for 30 min, then the residue should be resuspended in 0.9%-sodium chloride solution and centrifuged under the same conditions three or four times, then the residue should be supplemented with 0.1%-formalin solution up to initial volume and a stabilizer at the quantity of 0.1 ml/ml of the obtained diagnostic kit. Stabilizer should be chosen out of the group: 1.5%-polyvinylpyrrolidone and 7%-saccharose solution at the ratio of 1:1 or 0.03%-carbonate buffer and 3%-saccharose solution at the ratio of 1:1. Then diagnostic kit should be lyophilized. The present innovation enables to obtain desired diagnostic kit of higher specificity, wider sphere of application (agglutination micro-reaction, tube agglutination reaction and lamellar agglutination reaction upon a slide), more prolonged validity terms and higher convenience in usage.

EFFECT: higher simplicity and availability.

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