METHOD FOR PRODUCING ANTIGEN PREPARATION OF L-BRUCELLAS Russian patent published in 2011 - IPC A61K39/10 G01N33/531 

FIELD: medicine.

SUBSTANCE: invention refers to medicine and veterinary science, namely to microbiology and immunology. The method involves cultivation of Brucella abortus I-206 L-strain on a dense nutrient medium for L-brucellas at 37°C for 3-5 days. Thereafter, a microbial mass is washed of with buffered 0.9% normal saline of pH 7.2±0.2 and inactivated by adding 2.5% formalin. A bacterial suspension is kept for one day at 37°C with specific sterility being controlled. The bacterial suspension is reduced to concentration 4.5·10¹⁰-5·10 m.c. in ml with buffered 0.9% normal saline (pH 7.2±0.2) and thermally treated on a water bath at 100°C for 45-50 minutes. The suspension is centrifuged at 7000 rpm for 50-60 minutes; the supernatant is separated and frozen-dried. The method allows producing the preparation exhibiting high specificity and activity, available for preparing based immunobiological preparations and a test-system for human and animal blood serum examination for L-brucella antibodies.

EFFECT: method is technological, accessible, does not require using expensive devices and equipment.

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