METHOD OF ANTIGEN PREPARING FOR BRUCELLOSIS DIAGNOSIS Russian patent published in 1999 - IPC

Abstract RU 2133470 C1

FIELD: microbiology, biotechnology, medicine. SUBSTANCE: invention relates to producing preparations used for brucellosis diagnosis. Vaccine strain Brucella abortus-19 is cultured on the solid nutrient medium, the grown culture is washed off with citrate buffer (pH 5.3-5.5) and bacterial cells are inactivated by heating. Then the suspension of inactivated bacteria is resuspended with 12% sodium chloride solution to final concentration 2 x 10¹⁰ CFU/ml and dye methylene blue and preserving agent phenol are added. Obtained diagnosticum is used in serological agglutination reactions by Right and Heddlsone. EFFECT: decreased number of nonspecific serological reactions in brucellosis diagnosis, retained high sensitivity. 1 ex

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RU 2 133 470 C1

Authors

Ljapustina L.V.

Ljamkin G.I.

Dal'Vadjants V.G.

Taran I.F.

Dates

1999-07-20—Published

1997-07-31—Filed