METHOD FOR ION-EXCHANGE SEPARATION OF MIXTURE OF PHENYLALANINE AND TYROSINE Russian patent published in 2006 - IPC C07C227/40 C07C229/34 

FIELD: amino acids, chemical technology.

SUBSTANCE: invention relates to methods for separation and isolation of individual aromatic amino acids from their mixtures. Proposed method is carried out by multiple-stage procedure by continuous passing the parent solution of mixture of phenylalanine and tyrosine through column packed with strong-base anion-exchange resin AV-17-2P in OH-form and in periodic change of temperature regimen. After saturation of sorbent with amino acids the process is carried out by turns at two temperature values by collecting two fractions in outlet. At value T₁ = 20°C eluate is enriched with tyrosine as one component of the solution and at value T₂ = 80°C - with another component, i. e. phenylalanine. By changing volumes of fractions colleted at different values of temperature the method provides, if necessary, to enhancing degree of purification of one component of the mixture. Process can be carried out both in mono- and multi-stage regimen and both different columns and a single the same column can be used as different stages. Proposed invention provides the separation method that results to the enhanced effectiveness for separation of mixture of phenylalanine and tyrosine and excluding accessory reagent from the technological process used in regeneration and conversion of ion-exchange resins to working form, elimination of flows and enhanced yield of amino acids. Invention can be used in chemical, microbiological, food processing industry and agriculture.

EFFECT: improved separating method.

4 cl, 6 tbl, 4 ex