METHOD FOR ERYTHROCYTE FRACTIONATION IN DENSITY GRADIENT OF AGAROSE SOLUTION Russian patent published in 2006 - IPC G01N33/555 G01N33/559 

FIELD: medicine, biology, in particular method for erythrocyte fractionation.

SUBSTANCE: claimed method includes layering of erythrocyte mass on density gradient; centrifugation, light fraction-containing solution separation; centrifugation, mean fraction-containing solution separation from heavy fraction-containing precipitate, followed by purification of each fractions from density gradient residues. Erythrocyte mass is applied on 1 % agarose solution. To separate light fraction centrifugation is carried out at 5000 g; precipitate is layered on 1.5 % agarose solution; and to separate mean fraction from heavy fraction centrifugation is carried out at 6000 g. Each fraction is purified using chromatography column with glass filter.

EFFECT: accelerated and inexpensive method for production of three erythrocyte fraction fully purified from density gradient.

1 ex, 1 tbl, 1 dwg