FIELD: immunology.
SUBSTANCE: method involves selection of diluting sera by analysis of donor sera by immunoenzyme analysis and polymerase chain reaction for the presence of hepatitis B virus DNA, anti-HBs antibodies and factors of nonspecific binding HBsAg. Method involves selection of sera with negative result in these methods as diluting sera, titration of highly concentrated HBsAg preparations in diluting stabilized solution to the required concentration HBsAg (5-10 IU/ml) of AY and AD subtypes. Invention provides preparing two working serum standards comprising AY and AD subtypes of HBsAg retaining specific indices of these samples for a long time in storage at from 2°C to 6°C. Invention can be used in technology for preparing control samples for test-systems for detection of HBsAg.
EFFECT: improved preparing method.
4 cl, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING CONTROL PANEL FOR SERA AY AD OF SUBTYPES HBsAg FOR QUALITY CONTROL IN DIAGNOSIS OF HEPATITIS B | 2003 |
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| METHOD FOR PRODUCING BLOOD SERUM CONTROL PANELS FOR CONTROLLING HEPATITIS B DIAGNOSIS QUALITY | 1999 |
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RU2367960C2 |
