METHOD FOR PREPARING WEAKLY POSITIVE REFERENCE SERUM CONTAINING AD AND AY SUBTYPES OF HBsAg Russian patent published in 2013 - IPC G01N33/531 G01N33/576 

FIELD: medicine.

SUBSTANCE: invention represents a method for preparing weakly positive reference serum containing AD and AY subtypes of HBsAg involving donor serum sampling with ELISA, titration of the positive serum containing AD & AY subtypes of HBsAg in a dissolving stabilised solution, genotyping, thermal degradation test for establishing panel shelf life, differing by the fact that the positive reference serums containing AY and AD subtypes of HBsAg are dissolved to the range of the concentrations of 0.05 to 0.025 IU/ml and viscosity equal to that of normal human serum; and the dissolving stabilised solution is presented by negative defibrinated and lipid-free serum containing no markers of HBV infection which is to be detected by the test system of interest.

EFFECT: invention provides detection of HBV infection at the earlier stage of the disease and enables creating and controlling the test system quality.

5 cl, 2 tbl, 1 ex