FIELD: biotechnology, microbiology.
SUBSTANCE: method involves the complex use of native yeast autolyzate as a component of nutrient and protective media for lyophilization and taken in the amount 5-25% of the bacterial suspension volume. Protective medium comprises defatted milk and native yeast autolyzate taken in the ratio = 1:(0.5-1). Invention provides standardization of raw and process in preparing nutrient and protective media, reducing cost in preparing probiotics and enhances effectiveness of their production. Invention can be used in producing probiotic preparations: lactobacterin, bifidumbacterin, acilact and others.
EFFECT: improved preparing method.
2 cl, 1 tbl, 2 ex
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