FIELD: genetic engineering, biochemistry.
SUBSTANCE: method involves insertion of functional or nonfunctional sequence of nucleic acid encoding polypeptide that shows activity of enzyme desaturase into genome that encodes the plant oil or microorganism. After culturing the transformed organism in suitable conditions oil or triglyceride is isolated from this organism. Enhanced or diminished production of polypeptide in organism with activity of the corresponding desaturase results to change of the amount of fatty acid in it and with the new content of calendulic acid in it. Invention can be used in production of saturated or unsaturated fatty acids or triglycerides with the enhanced content of such acids.
EFFECT: improved and valuable properties of gene.
8 cl, 7 dwg, 6 ex
