DELTA-5-DESATURASE AND ITS USE FOR PRODUCING POLYUNSATURATED FATTY ACIDS Russian patent published in 2012 - IPC C12N15/53 C12N9/02 C12N15/11 C12P7/64 

FIELD: medicine.

SUBSTANCE: what is presented is a recovered polynucleotide for expression of delta-5-desaturase containing a nucleotide sequence: 1) coding a polypeptide of delta-5-desaturase with the polypeptide having at least 80% identity SEQ ID NO:2; 2) coding the polypeptide of delta-5-desaturase with the nucleotide sequence having at least 90% identity SEQ ID NO:1 ot SEQ ID NO:3; 3) coding the polypeptide of delta-5-desaturase with the nucleotide sequence being shock-hybridised with the nucleotide sequence presented in SEQ ID NO:1 or SEQ ID NO:3; 4) a complement of the nucleotide sequence 1)-3). There are described a recombinant DNA structure, a host cell, an oilseed transgenic plant, a transgenic seed containing said polynucleotide. The following methods are presented: host cell transformation, expression of long-chain polyunsaturated fatty acids in a plant cell, production of at least one polyunsaturated fatty acid in a plant with the use of said polypeptide. Usability of said transgenic seeds for producing human foodstuff, animal feed, oil and lecithin is described.

EFFECT: invention enables producing a changed profile of polyunsaturated fatty acids as compared with a profile of polyunsaturated fatty acids of a non-transformed plant.

24 cl, 11 tbl, 22 dwg, 20 ex