FIELD: chemistry.
SUBSTANCE: invention relates to the field of biotechnology. Claimed are version proteins of lysophospholipid-acyltransferase, catalysing a reaction of converting 18:3(n-6)-PL into 18:3(n-6)-CoA and/or DGLA-CoA into DGLA-PL, which have amino acid sequences, at least, by 95% identical to SEQ ID NO:2 and 7, given in the description. In addition, nucleic acids, coding the said proteins, are disclosed. Also claimed are: a recombinant expression vector, containing the said nucleic acids, and a host cell for obtaining a composition of fatty acids, transformed with the said vector. Claimed is the application of the said vector for increasing a quantitative ratio of arachidonic acid (ARA) in the composition of fatty acids in the host. Described is a method of obtaining the composition of fatty acids, which includes cultivation of the host cell for obtaining the composition of fatty acids with the increased quantity of arachidonic acid in comparison with its quantity in the composition of fatty acids, obtained by cultivation of a non-transformed host, and the collection of the composition of fatty acids from cultures of the transformed cell.
EFFECT: invention makes it possible to obtain the composition of fatty acids with an increased content of arachidonic acid.
18 cl, 8 dwg, 8 tbl, 3 ex
