FIELD: biology, immunology.
SUBSTANCE: method involves preparing a stabilizing solution for providing the maximal retention of isolated viable blood leukocytes and prevention of their sticking. Method involves taking off a blood sample and isolation of leukocytes by using the concentrate Haenx without phenol red that is used in the ratio with water = 1:9 and containing additionally glucose, lyophilized bovine serum albumin in the dose 10-15 mg per 100 ml of each. Method provides retaining the constant parameters of cells and to enhance retention period of viable leukocytes (90-95%) in carrying out the chemiluminescent analysis.
EFFECT: improved retaining method.
3 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ISOLATING BLOOD LEUKOCYTES FOR CHEMILUMINESCENT ANALYSIS | 2002 |
|
RU2232395C2 |
| METHOD OF LIFE-TIME EXCLUSION OF TUBERCULOSIS | 2008 |
|
RU2389020C2 |
| METHOD FOR INTRAVITAM PREDICTION OF TUBERCULOSIS | 2004 |
|
RU2296334C2 |
| METHOD OF LIFE-TIME DIAGNOSIS OF CATTLE TUBERCULOSIS | 2020 |
|
RU2738133C1 |
| METHOD FOR KEEPING VIABLE BLOOD CELLS FOR PURPOSES OF CHEMOLUMINESCENT ANALYSIS | 2010 |
|
RU2456595C2 |
| POLYFUNCTIONAL CHEMILUMINESCENT COMPOSITION | 2008 |
|
RU2378315C1 |
| METHOD OF PREPARING SUBSTANCE SHOWING IMMUNOSTIMULATING, ANTIVIRAL AND ANTIBACTERIAL ACTIVITY, SUBSTANCE OBTAINED BY THIS METHOD AND PHARMACEUTICAL COMPOSITION BASED ON THEREOF | 2001 |
|
RU2195308C1 |
| PRESERVING AGENT FOR TRANSPLANTABLE CELLS STORAGE | 1991 |
|
RU2036234C1 |
| METHOD OF INDIVIDUAL EVALUATION OF SENSITIVITY TO HEMOCONTACT PREPARATIONS FOR HEMOSORPTION PROCEDURES IN PATIENTS | 1997 |
|
RU2122734C1 |
| METHOD OF PRODUCING CATTLE EMBRYOS IN VITRO | 2023 |
|
RU2818068C1 |
