FIELD: biotechnology, in particular production of modified swine factor VIII (POL1212).
SUBSTANCE: DNA molecule encoding of modified swine factor VIII is cloned in expression vector, having functionality in mammalian cells. Modified swine factor VIII protein is obtained by cultivation of mammalian cell line BHK CRL-1632 (ATCC), BHK 1632, or CHO-K1, transfected with vector. Therapeutic composition for treatment of subjects suffering from deficit of factor VIII, such as haemophilia, contains effective amount of swine factor VIII protein.
EFFECT: effective agent for treatment of factor VIII deficit.
13 cl, 8 dwg, 7 ex
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