FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology, namely to obtaining B-domain truncated factor VIII molecule and covalently conjugated with hydrophilic polymer, which has altered half-life in bloodstream, and can be used in medicine to treat haemophilia. Molecule of precursor of B-domain truncated factor VII, where B-domain corresponds to amino acids 741-761 from SEQ ID NO 2, is obtained. Said molecule is covalently conjugated with PEG or polysaccharide with size 10-80 kDa by means of O-linked oligosaccharide at serine residue in truncated B-domain, which corresponds to amino acid 750 in SEQ ID NO 2.
EFFECT: invention makes it possible to increase factor VIII half-life in bloodstream.
8 cl, 8 dwg, 4 tbl, 7 ex
| Title | Year | Author | Number |
|---|---|---|---|
| FVIII MODIFICATION TREND SITE | 2005 |
|
RU2423380C2 |
| PURIFICATION OF FACTOR VIII SUBTYPES | 2018 |
|
RU2800431C2 |
| RECOMBINANT PLASMID DNA pAP227 CODING POLYPEPTIDE OF RECOMBINANT FACTOR VIII OF HUMAN BLOOD COAGULABILITY, LINE OF CELLS Cricetulus griseus CHO, 2H5-PRODUCER OF RECOMBINANT FACTOR VIII OF HUMAN BLOOD COAGULABILITY, AND METHOD FOR OBTAINING POLYPEPTIDE HAVING ACTIVITY OF RECOMBINANT FACTOR VIII | 2012 |
|
RU2500818C1 |
| EXPRESSION GENETIC MAKER pOptivec/F8BDD CODING HUMAN RECOMBINANT BLOOD COAGULABILITY FACTOR VIII WITH DELETION OF B-DOMAIN AND CELL LINE DG-OV-F8BDD-18 PRODUCING HUMAN RECOMBINANT BLOOD COAGULABILITY FACTOR VIII WITH DELETION OF B-DOMAIN | 2009 |
|
RU2429294C2 |
| PREPARATIONS CONTAINING VON WILLEBRAND FACTOR (vWF) AND METHODS, KITS AND APPLICATIONS RELATED TO THEM | 2010 |
|
RU2579977C2 |
| CHEMERIC IMPROVED RECOMBINANT FACTOR VIII | 2015 |
|
RU2647769C2 |
| IMPROVED FVIII FUSION PROTEIN AND ITS USE | 2019 |
|
RU2789085C2 |
| HIGH GLYCATED FUSED PROTEIN BASED ON HUMAN BLOOD COAGULATION FACTOR VIII, METHOD FOR PRODUCTION THEREOF AND USE THEREOF | 2016 |
|
RU2722374C1 |
| CONJUGATES OF ERYTHROPOIETIN AND POLYETHYLENE GLYCOL, PHARMACEUTICAL COMPOSITIONS (VARIANTS), METHOD FOR PROPHYLACTIC AND/OR THERAPEUTIC TREATMENT OF DISTURBANCES AND METHOD FOR PREPARING CONJUGATE OR COMPOSITION | 2000 |
|
RU2232163C2 |
| EXPRESSION PLASMID FOR HUMAN RECOMBINANT COAGULATION FACTOR VIII WITH DELETED B-DOMAIN IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT COAGULATION FACTOR VIII WITH DELETED B-DOMAIN, AND METHOD FOR PRODUCING ABOVE FACTOR | 2013 |
|
RU2561466C2 |
